bwa mem genome.fa sample_R1.fastq sample_R2.fastq > aligned.sam samtools sort -@8 aligned.sam -o sorted.bam freebayes -f genome.fa sorted.bam > variants.vcf Then I wait. This is when I practice patience. And refresh my email 47 times.
The hardest part of my job isn’t the code—it’s the interpretation . david bioinfo
My day doesn’t start with a microscope. It starts with a terminal. Usually, I’m staring at a 50GB FASTQ file that decided to corrupt itself at 2:00 AM. bwa mem genome
You can have the cleanest pipeline, the most parallelized code, and a server with 1TB of RAM. But if you don’t understand the biological question, you’re just moving bytes around. The hardest part of my job isn’t the
Sometimes, I’m a plumber (unclogging data pipelines). Sometimes, a detective (finding a single SNP in 3 billion base pairs). And once a month, I’m a philosopher (arguing whether a p-value of 0.051 is really non-significant).